Discover the affects of PH on the enzyme catalase

Catalase- This is an enzyme found in food such as white potato vine and liver. It is utilize for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. It speeds up the decomposition of atomic number 1 Peroxide into oxygen and water. It is able to do this be political campaign the variant of its active billet matches the shape of Hydrogen Peroxide pinch. This type of reception where a molecule is broken down into smaller pieces is an ANABOLIC REACTION. undercoat INFORMATION ON ENZYMESEnzymes- Enzymes are complex 3-D globular proteins, round of which have other associated molecules. Enzymes are a catalyst which alters the rate of chemic reaction with prohibited itself undergoing a permanent change, and therefore can be utilise over and over again.Enzymes help reactions speed up which would otherwise pull back place very slowly.While the enzyme molecule is relatively large than the larger than the substratum molecule it acts upon , lone(prenominal) a small part of the enzyme molecule actually comes into contact. This region is called the ACTIVE SITE. The active site of an enzyme is the region that binds the substrate and contributes the amino acid residues that directly participate in the making and intermission of chemical bonds. However, all enzymes operate only on a particularised shape and therefore fit only complementary locks, so only subst rank of a particular shape give fit the active site of an enzyme.PredictionI predict that as the PH increases the activity of enzyme increases until the best PH is reached. Catalase optimum ph is around 6-7.after that the enzyme will be denatured. possibilityThe enzyme will have an optimum pH of around neutral. Either spot of the optimum pH will have relatively high rates of reaction or far away from the optimum pH will have a low rate of reaction. The enzyme catalase is found in legion(predicate) tissues including potato and liver. It is important because i t breaks down the hydrogen peroxide formed in metabolism. Hydrogen peroxide is toxic and if it were not broken down it would kill the cells. As it does this, it liberates carbon dioxide that can be collected.CHEMICAL EQUATION2H2O2 - 2H2O + O2I also hypothesize that the catalase activity will be highest with the sample of potato comparing to the apple.VariablesThe factors, which will have an affect on the enzyme activity, will be* Temperature* Concentration of enzymes* pHThe variable in which I will be using to investigate the enzyme activity is the temperature. The rest of the variables will be unploughed constant and only the temperatures will be changed.ApparatusTo test how the PH affects the rate of reaction, I will use the following apparatus* hitman syringe through bung* Potato* Hydrogen Peroxide* collar Clock* Cork borer* Scalpel* water Beaker-250 cm* Delivery tubing* Ph 3,5,7,9* 5 cm or 10 cm malleable syringe with end cut off* measuring cylinder* conical flask* White tile* wax* Test pipings* Test tube rack* Safety equipment goggles, lab coat, glovesAlternativeUse yeast, broad beans or liver, instead of potato and apple.Method1. Mea genuine out 5cm3 of potato into a conical flask2. Mea true out 10 cm3 of buffer dissolver into a separate container and mix it with minced potato.3. Measure out 5cm3 volume of hydrogen peroxide in syringe.4. Fill a tub with water.5. Completely accept a measuring cylinder in the water making sure to release all air bubbles in the cylinder and keep underwater.6. stupefy the cylinder upright ensuring the open end remains underwater and fix securely7. Add the hydrogen peroxide to the potato and place bung on conical flask whilst starting timer.8. Run experiment for 5 minutes taking readings every 30 seconds by noting the amount of botch up at top of cylinder (at meniscus)9. Repeat the experiment with different ph.apology of methodI have chosen to use this method because it is safe, efficacious and accurate. I feel that it will help me to obtain dependable results. jeopardize Assessment* Hydrogen peroxide is a dangerous substance. It can cause serious injury to the eyes and skin. It is therefore necessary that goggles be used for eye protection. In addition, eye irrigation equipment should be readily addressable in case of an emergency.* The work area must be clean, clear and dry. Make sure there is no obstacle in the working area so that you can easily move around as. This allows you to coiffe the experiment safely and unimpeded care should be taken to visualize that liquid is not spilled onto the floor to cause slippage.Result tableTime(s) oxygen gas collected (cm3)Ph 3 ph 5 ph7 ph930 1.5 1.5 0.5 1.560 1.5 3.0 1.5 2.090 1.5 5.0 2.0 2.0120 1.5 7.0 5.65 2.0150 1.5 8.3 10.3 2.0180 1.5 9.0 14.35 2.0210 1.5 10.0 17.35 2.0240 1.5 11.1 20.15 2.0270 1.5 13.6 24.15 2.0300 1.5 14.0 31.15 2.0These results clear up that the optimum ph for catalase is 7 which supports my prediction. At the lowest ph 3, slight oxygen was released, measuring a slower rate of reaction. My background nurture explains this. The enzyme dont work as it is neared extreme PHS. This is because its shape would have been change sparingly means the active site may not fit into the substrate any more.Further investigationIf this investigation were to be done again, I would take all results myself and use my own interpretation, thus gaining more reliable results. I will know how much potato to measure out for each experiment and also I will make sure that the other variables are kept constant. I have taken the alike(p) amount of each, keeping them constant. This would give me clearer results to aid me in my understanding as to how ph can affect the activity of catalase.